
CCP4 Roadmaps 
EXPERIMENTAL PHASING 
MULTIPLE ISOMORPHOUS REPLACEMENT AND MULTIWAVELENGTH ANOMALOUS DISPERSION
The plain rectangular boxes represent
tasks as in the
CCP4 Graphical User Interface, the steps to take for experimental phasing.
Stippled rectangular boxes represent external programs. 'Difference Fourier' is not part of the
Experimental Phasing module
in the Interface; for this,
Run FFT  Create Map
is used, in the
Map & Mask Utilities module.
The ellipsoids represent the input and output to and from the various tasks.
Auxiliary CCP4 programs used in these tasks
 CROSSEC
 Lest anomalous scattering factors f' and f" at various Xray wavelengths
 FINDNCS
 Detect NCS operations automatically from heavy atom sites
 MTZMADMOD
 Generate F^{+}/F^{} or F/D from other for anomalous data
 OASIS
 Breaking phase ambiguity in OAS or SIR
 PEAKMAX
 Search for peaks in the electron density map
 VECREF
 Vectorspace refinement of heavy atom sites in isomorphous derivatives
 VECSUM
 Program to deconvolute a Patterson function and solve the structure (unsupported)
 VECTORS
 Generates Patterson vectors from atomic coordinates
Other nonCCP4 programs
 AD@LLNL
 Anomalous scattering coefficients  forms interface to calculate expected anomalous dispersion ratios
 AS@UW
 Anomalous scattering coefficients  forms interface to plot theoretical values of f' and f''
 CHOOCH
 Calculating Anomalous Scattering Factors from Xray fluorescence data
 SharpHome@MRC
 Statistical HeavyAtom Refinement and Phasing
 SnB@SUNY
 A DirectMethods Procedure for Determining Crystal Structures
 SOLVE@LANL
 Automated crystallographic structure solution for MIR and MAD
Further reading
CCP4i Documentation on Experimental Phasing
Tutorial on Isomorphous
Replacement and Anomalous Scattering (I. Tickle)
ScaleChoose
MAD Phasing
CCP4 Program Documentation
